dapi reagent Search Results


prolong gold antifade reagent with dapi  (Cell Signaling Technology Inc)
97
Cell Signaling Technology Inc prolong gold antifade reagent with dapi
Fig. 3 CX-5461 causes DNA damage and cell death by apoptosis in neuroblastoma cells at sub-micromolar concentrations. a Apoptosis in CHP-134 cells. The cells were treated for 48 h. b Quantification of data in (a). Data represent the mean ± SD of three independent experiments. Significant P-values (left to right): 3.9 × 10−6, 7.2 × 10−7, and 4.8 × 10−7. c Quantification of flow cytometry data showing apoptosis up to 48 h in CHP-134 and IMR-5 (0.2 µM and 0.1 µM of CX-5461 respectively). CHP-134 cells were treated with 0.2 µM and IMR-5 cells with 0.1 µM of CX-5461. Data represent the mean ± SD of three independent experiments. Significant P-values (left to right): CHP-134, 2.2 × 10−4, 3.2 × 10−5, and 1.5 × 10−5; IMR-5, 1.8 × 10−4, 2.3 × 10−5, and 2.5 × 10−6. d Western blots for cleaved PARP (c-PARP). β-Actin was used as a loading control. Representative results of three biological replicates are shown. e Co-localization of 53BP1 and γ-H2AX. Cells were treated for 24 h. Representative results of three biological replicates are shown. Scale bar = 10 μm. See Fig. S3d for more time points. f Comet assay in CHP-134 cells. Tail length (left) and representative images (right) are shown. The red line indicates the mean value of tail length in each group. Scale bar = 5 µm. g Cell cycle analysis forCHP-134 (0.2 µM CX-5461) and IMR-5 (0.05 µM CX-5461) cells. Data represent the mean ± SD of three independent experiments. P-values vs 0 h: P1 = 4.2 × 10−5, P2 = 7.1 × 10−7, P3 = 6.3 × 10−5, P4 = 2.7 × 10−4, P5 = 6.3 × 10−3, P6 = 2.3 × 10−5, P7 = 1.8 × 10−4, and P8 = 0.044. h EdU incorporation assay to assess global nascent DNA transcription. Representative results of three biological replicates are shown. Blue, <t>DAPI;</t> green, EdU. Scale bar = 20 µm. i CX-5461 induced DNA damage (γ-H2AX, red) was enriched in cells with active DNA synthesis (positive for EdU (green) labeling). Representative results of three biological replicates are shown. Scale bar = 10 μm. Two-tailed t- test was used for (b, c, g). Source data for these panels are included in Source Data file.
Prolong Gold Antifade Reagent With Dapi, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/prolong gold antifade reagent with dapi/product/Cell Signaling Technology Inc
Average 97 stars, based on 1 article reviews
prolong gold antifade reagent with dapi - by Bioz Stars, 2026-04
97/100 stars
  Buy from Supplier
dapi and polyt staining reagent  (Vizgen Inc)
90
Vizgen Inc dapi and polyt staining reagent
Fig. 3 CX-5461 causes DNA damage and cell death by apoptosis in neuroblastoma cells at sub-micromolar concentrations. a Apoptosis in CHP-134 cells. The cells were treated for 48 h. b Quantification of data in (a). Data represent the mean ± SD of three independent experiments. Significant P-values (left to right): 3.9 × 10−6, 7.2 × 10−7, and 4.8 × 10−7. c Quantification of flow cytometry data showing apoptosis up to 48 h in CHP-134 and IMR-5 (0.2 µM and 0.1 µM of CX-5461 respectively). CHP-134 cells were treated with 0.2 µM and IMR-5 cells with 0.1 µM of CX-5461. Data represent the mean ± SD of three independent experiments. Significant P-values (left to right): CHP-134, 2.2 × 10−4, 3.2 × 10−5, and 1.5 × 10−5; IMR-5, 1.8 × 10−4, 2.3 × 10−5, and 2.5 × 10−6. d Western blots for cleaved PARP (c-PARP). β-Actin was used as a loading control. Representative results of three biological replicates are shown. e Co-localization of 53BP1 and γ-H2AX. Cells were treated for 24 h. Representative results of three biological replicates are shown. Scale bar = 10 μm. See Fig. S3d for more time points. f Comet assay in CHP-134 cells. Tail length (left) and representative images (right) are shown. The red line indicates the mean value of tail length in each group. Scale bar = 5 µm. g Cell cycle analysis forCHP-134 (0.2 µM CX-5461) and IMR-5 (0.05 µM CX-5461) cells. Data represent the mean ± SD of three independent experiments. P-values vs 0 h: P1 = 4.2 × 10−5, P2 = 7.1 × 10−7, P3 = 6.3 × 10−5, P4 = 2.7 × 10−4, P5 = 6.3 × 10−3, P6 = 2.3 × 10−5, P7 = 1.8 × 10−4, and P8 = 0.044. h EdU incorporation assay to assess global nascent DNA transcription. Representative results of three biological replicates are shown. Blue, <t>DAPI;</t> green, EdU. Scale bar = 20 µm. i CX-5461 induced DNA damage (γ-H2AX, red) was enriched in cells with active DNA synthesis (positive for EdU (green) labeling). Representative results of three biological replicates are shown. Scale bar = 10 μm. Two-tailed t- test was used for (b, c, g). Source data for these panels are included in Source Data file.
Dapi And Polyt Staining Reagent, supplied by Vizgen Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dapi and polyt staining reagent/product/Vizgen Inc
Average 90 stars, based on 1 article reviews
dapi and polyt staining reagent - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
dapi fluorescent sealing reagent  (Elabscience Biotechnology)
90
Elabscience Biotechnology dapi fluorescent sealing reagent
Fig. 3 CX-5461 causes DNA damage and cell death by apoptosis in neuroblastoma cells at sub-micromolar concentrations. a Apoptosis in CHP-134 cells. The cells were treated for 48 h. b Quantification of data in (a). Data represent the mean ± SD of three independent experiments. Significant P-values (left to right): 3.9 × 10−6, 7.2 × 10−7, and 4.8 × 10−7. c Quantification of flow cytometry data showing apoptosis up to 48 h in CHP-134 and IMR-5 (0.2 µM and 0.1 µM of CX-5461 respectively). CHP-134 cells were treated with 0.2 µM and IMR-5 cells with 0.1 µM of CX-5461. Data represent the mean ± SD of three independent experiments. Significant P-values (left to right): CHP-134, 2.2 × 10−4, 3.2 × 10−5, and 1.5 × 10−5; IMR-5, 1.8 × 10−4, 2.3 × 10−5, and 2.5 × 10−6. d Western blots for cleaved PARP (c-PARP). β-Actin was used as a loading control. Representative results of three biological replicates are shown. e Co-localization of 53BP1 and γ-H2AX. Cells were treated for 24 h. Representative results of three biological replicates are shown. Scale bar = 10 μm. See Fig. S3d for more time points. f Comet assay in CHP-134 cells. Tail length (left) and representative images (right) are shown. The red line indicates the mean value of tail length in each group. Scale bar = 5 µm. g Cell cycle analysis forCHP-134 (0.2 µM CX-5461) and IMR-5 (0.05 µM CX-5461) cells. Data represent the mean ± SD of three independent experiments. P-values vs 0 h: P1 = 4.2 × 10−5, P2 = 7.1 × 10−7, P3 = 6.3 × 10−5, P4 = 2.7 × 10−4, P5 = 6.3 × 10−3, P6 = 2.3 × 10−5, P7 = 1.8 × 10−4, and P8 = 0.044. h EdU incorporation assay to assess global nascent DNA transcription. Representative results of three biological replicates are shown. Blue, <t>DAPI;</t> green, EdU. Scale bar = 20 µm. i CX-5461 induced DNA damage (γ-H2AX, red) was enriched in cells with active DNA synthesis (positive for EdU (green) labeling). Representative results of three biological replicates are shown. Scale bar = 10 μm. Two-tailed t- test was used for (b, c, g). Source data for these panels are included in Source Data file.
Dapi Fluorescent Sealing Reagent, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dapi fluorescent sealing reagent/product/Elabscience Biotechnology
Average 90 stars, based on 1 article reviews
dapi fluorescent sealing reagent - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
prolong diamond antifade mounting solution  (Lifetech Scientific Corporation)
90
Lifetech Scientific Corporation prolong diamond antifade mounting solution
Fig. 3 CX-5461 causes DNA damage and cell death by apoptosis in neuroblastoma cells at sub-micromolar concentrations. a Apoptosis in CHP-134 cells. The cells were treated for 48 h. b Quantification of data in (a). Data represent the mean ± SD of three independent experiments. Significant P-values (left to right): 3.9 × 10−6, 7.2 × 10−7, and 4.8 × 10−7. c Quantification of flow cytometry data showing apoptosis up to 48 h in CHP-134 and IMR-5 (0.2 µM and 0.1 µM of CX-5461 respectively). CHP-134 cells were treated with 0.2 µM and IMR-5 cells with 0.1 µM of CX-5461. Data represent the mean ± SD of three independent experiments. Significant P-values (left to right): CHP-134, 2.2 × 10−4, 3.2 × 10−5, and 1.5 × 10−5; IMR-5, 1.8 × 10−4, 2.3 × 10−5, and 2.5 × 10−6. d Western blots for cleaved PARP (c-PARP). β-Actin was used as a loading control. Representative results of three biological replicates are shown. e Co-localization of 53BP1 and γ-H2AX. Cells were treated for 24 h. Representative results of three biological replicates are shown. Scale bar = 10 μm. See Fig. S3d for more time points. f Comet assay in CHP-134 cells. Tail length (left) and representative images (right) are shown. The red line indicates the mean value of tail length in each group. Scale bar = 5 µm. g Cell cycle analysis forCHP-134 (0.2 µM CX-5461) and IMR-5 (0.05 µM CX-5461) cells. Data represent the mean ± SD of three independent experiments. P-values vs 0 h: P1 = 4.2 × 10−5, P2 = 7.1 × 10−7, P3 = 6.3 × 10−5, P4 = 2.7 × 10−4, P5 = 6.3 × 10−3, P6 = 2.3 × 10−5, P7 = 1.8 × 10−4, and P8 = 0.044. h EdU incorporation assay to assess global nascent DNA transcription. Representative results of three biological replicates are shown. Blue, <t>DAPI;</t> green, EdU. Scale bar = 20 µm. i CX-5461 induced DNA damage (γ-H2AX, red) was enriched in cells with active DNA synthesis (positive for EdU (green) labeling). Representative results of three biological replicates are shown. Scale bar = 10 μm. Two-tailed t- test was used for (b, c, g). Source data for these panels are included in Source Data file.
Prolong Diamond Antifade Mounting Solution, supplied by Lifetech Scientific Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/prolong diamond antifade mounting solution/product/Lifetech Scientific Corporation
Average 90 stars, based on 1 article reviews
prolong diamond antifade mounting solution - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
prolong anti-fade  (Promega)
90
Promega prolong anti-fade
Fig. 3 CX-5461 causes DNA damage and cell death by apoptosis in neuroblastoma cells at sub-micromolar concentrations. a Apoptosis in CHP-134 cells. The cells were treated for 48 h. b Quantification of data in (a). Data represent the mean ± SD of three independent experiments. Significant P-values (left to right): 3.9 × 10−6, 7.2 × 10−7, and 4.8 × 10−7. c Quantification of flow cytometry data showing apoptosis up to 48 h in CHP-134 and IMR-5 (0.2 µM and 0.1 µM of CX-5461 respectively). CHP-134 cells were treated with 0.2 µM and IMR-5 cells with 0.1 µM of CX-5461. Data represent the mean ± SD of three independent experiments. Significant P-values (left to right): CHP-134, 2.2 × 10−4, 3.2 × 10−5, and 1.5 × 10−5; IMR-5, 1.8 × 10−4, 2.3 × 10−5, and 2.5 × 10−6. d Western blots for cleaved PARP (c-PARP). β-Actin was used as a loading control. Representative results of three biological replicates are shown. e Co-localization of 53BP1 and γ-H2AX. Cells were treated for 24 h. Representative results of three biological replicates are shown. Scale bar = 10 μm. See Fig. S3d for more time points. f Comet assay in CHP-134 cells. Tail length (left) and representative images (right) are shown. The red line indicates the mean value of tail length in each group. Scale bar = 5 µm. g Cell cycle analysis forCHP-134 (0.2 µM CX-5461) and IMR-5 (0.05 µM CX-5461) cells. Data represent the mean ± SD of three independent experiments. P-values vs 0 h: P1 = 4.2 × 10−5, P2 = 7.1 × 10−7, P3 = 6.3 × 10−5, P4 = 2.7 × 10−4, P5 = 6.3 × 10−3, P6 = 2.3 × 10−5, P7 = 1.8 × 10−4, and P8 = 0.044. h EdU incorporation assay to assess global nascent DNA transcription. Representative results of three biological replicates are shown. Blue, <t>DAPI;</t> green, EdU. Scale bar = 20 µm. i CX-5461 induced DNA damage (γ-H2AX, red) was enriched in cells with active DNA synthesis (positive for EdU (green) labeling). Representative results of three biological replicates are shown. Scale bar = 10 μm. Two-tailed t- test was used for (b, c, g). Source data for these panels are included in Source Data file.
Prolong Anti Fade, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/prolong anti-fade/product/Promega
Average 90 stars, based on 1 article reviews
prolong anti-fade - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
ao-dapi staining reagent solution 18  (ChemoMetec)
90
ChemoMetec ao-dapi staining reagent solution 18
Fig. 3 CX-5461 causes DNA damage and cell death by apoptosis in neuroblastoma cells at sub-micromolar concentrations. a Apoptosis in CHP-134 cells. The cells were treated for 48 h. b Quantification of data in (a). Data represent the mean ± SD of three independent experiments. Significant P-values (left to right): 3.9 × 10−6, 7.2 × 10−7, and 4.8 × 10−7. c Quantification of flow cytometry data showing apoptosis up to 48 h in CHP-134 and IMR-5 (0.2 µM and 0.1 µM of CX-5461 respectively). CHP-134 cells were treated with 0.2 µM and IMR-5 cells with 0.1 µM of CX-5461. Data represent the mean ± SD of three independent experiments. Significant P-values (left to right): CHP-134, 2.2 × 10−4, 3.2 × 10−5, and 1.5 × 10−5; IMR-5, 1.8 × 10−4, 2.3 × 10−5, and 2.5 × 10−6. d Western blots for cleaved PARP (c-PARP). β-Actin was used as a loading control. Representative results of three biological replicates are shown. e Co-localization of 53BP1 and γ-H2AX. Cells were treated for 24 h. Representative results of three biological replicates are shown. Scale bar = 10 μm. See Fig. S3d for more time points. f Comet assay in CHP-134 cells. Tail length (left) and representative images (right) are shown. The red line indicates the mean value of tail length in each group. Scale bar = 5 µm. g Cell cycle analysis forCHP-134 (0.2 µM CX-5461) and IMR-5 (0.05 µM CX-5461) cells. Data represent the mean ± SD of three independent experiments. P-values vs 0 h: P1 = 4.2 × 10−5, P2 = 7.1 × 10−7, P3 = 6.3 × 10−5, P4 = 2.7 × 10−4, P5 = 6.3 × 10−3, P6 = 2.3 × 10−5, P7 = 1.8 × 10−4, and P8 = 0.044. h EdU incorporation assay to assess global nascent DNA transcription. Representative results of three biological replicates are shown. Blue, <t>DAPI;</t> green, EdU. Scale bar = 20 µm. i CX-5461 induced DNA damage (γ-H2AX, red) was enriched in cells with active DNA synthesis (positive for EdU (green) labeling). Representative results of three biological replicates are shown. Scale bar = 10 μm. Two-tailed t- test was used for (b, c, g). Source data for these panels are included in Source Data file.
Ao Dapi Staining Reagent Solution 18, supplied by ChemoMetec, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ao-dapi staining reagent solution 18/product/ChemoMetec
Average 90 stars, based on 1 article reviews
ao-dapi staining reagent solution 18 - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
antifade reagent 4’,6-diamidino-2-phenylindole (dapi)  (Beyotime)
90
Beyotime antifade reagent 4’,6-diamidino-2-phenylindole (dapi)
Fig. 3 CX-5461 causes DNA damage and cell death by apoptosis in neuroblastoma cells at sub-micromolar concentrations. a Apoptosis in CHP-134 cells. The cells were treated for 48 h. b Quantification of data in (a). Data represent the mean ± SD of three independent experiments. Significant P-values (left to right): 3.9 × 10−6, 7.2 × 10−7, and 4.8 × 10−7. c Quantification of flow cytometry data showing apoptosis up to 48 h in CHP-134 and IMR-5 (0.2 µM and 0.1 µM of CX-5461 respectively). CHP-134 cells were treated with 0.2 µM and IMR-5 cells with 0.1 µM of CX-5461. Data represent the mean ± SD of three independent experiments. Significant P-values (left to right): CHP-134, 2.2 × 10−4, 3.2 × 10−5, and 1.5 × 10−5; IMR-5, 1.8 × 10−4, 2.3 × 10−5, and 2.5 × 10−6. d Western blots for cleaved PARP (c-PARP). β-Actin was used as a loading control. Representative results of three biological replicates are shown. e Co-localization of 53BP1 and γ-H2AX. Cells were treated for 24 h. Representative results of three biological replicates are shown. Scale bar = 10 μm. See Fig. S3d for more time points. f Comet assay in CHP-134 cells. Tail length (left) and representative images (right) are shown. The red line indicates the mean value of tail length in each group. Scale bar = 5 µm. g Cell cycle analysis forCHP-134 (0.2 µM CX-5461) and IMR-5 (0.05 µM CX-5461) cells. Data represent the mean ± SD of three independent experiments. P-values vs 0 h: P1 = 4.2 × 10−5, P2 = 7.1 × 10−7, P3 = 6.3 × 10−5, P4 = 2.7 × 10−4, P5 = 6.3 × 10−3, P6 = 2.3 × 10−5, P7 = 1.8 × 10−4, and P8 = 0.044. h EdU incorporation assay to assess global nascent DNA transcription. Representative results of three biological replicates are shown. Blue, <t>DAPI;</t> green, EdU. Scale bar = 20 µm. i CX-5461 induced DNA damage (γ-H2AX, red) was enriched in cells with active DNA synthesis (positive for EdU (green) labeling). Representative results of three biological replicates are shown. Scale bar = 10 μm. Two-tailed t- test was used for (b, c, g). Source data for these panels are included in Source Data file.
Antifade Reagent 4’,6 Diamidino 2 Phenylindole (Dapi), supplied by Beyotime, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/antifade reagent 4’,6-diamidino-2-phenylindole (dapi)/product/Beyotime
Average 90 stars, based on 1 article reviews
antifade reagent 4’,6-diamidino-2-phenylindole (dapi) - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
dapi, cell viability and cytotoxicity assay reagents  (Beyotime)
90
Beyotime dapi, cell viability and cytotoxicity assay reagents
Fig. 3 CX-5461 causes DNA damage and cell death by apoptosis in neuroblastoma cells at sub-micromolar concentrations. a Apoptosis in CHP-134 cells. The cells were treated for 48 h. b Quantification of data in (a). Data represent the mean ± SD of three independent experiments. Significant P-values (left to right): 3.9 × 10−6, 7.2 × 10−7, and 4.8 × 10−7. c Quantification of flow cytometry data showing apoptosis up to 48 h in CHP-134 and IMR-5 (0.2 µM and 0.1 µM of CX-5461 respectively). CHP-134 cells were treated with 0.2 µM and IMR-5 cells with 0.1 µM of CX-5461. Data represent the mean ± SD of three independent experiments. Significant P-values (left to right): CHP-134, 2.2 × 10−4, 3.2 × 10−5, and 1.5 × 10−5; IMR-5, 1.8 × 10−4, 2.3 × 10−5, and 2.5 × 10−6. d Western blots for cleaved PARP (c-PARP). β-Actin was used as a loading control. Representative results of three biological replicates are shown. e Co-localization of 53BP1 and γ-H2AX. Cells were treated for 24 h. Representative results of three biological replicates are shown. Scale bar = 10 μm. See Fig. S3d for more time points. f Comet assay in CHP-134 cells. Tail length (left) and representative images (right) are shown. The red line indicates the mean value of tail length in each group. Scale bar = 5 µm. g Cell cycle analysis forCHP-134 (0.2 µM CX-5461) and IMR-5 (0.05 µM CX-5461) cells. Data represent the mean ± SD of three independent experiments. P-values vs 0 h: P1 = 4.2 × 10−5, P2 = 7.1 × 10−7, P3 = 6.3 × 10−5, P4 = 2.7 × 10−4, P5 = 6.3 × 10−3, P6 = 2.3 × 10−5, P7 = 1.8 × 10−4, and P8 = 0.044. h EdU incorporation assay to assess global nascent DNA transcription. Representative results of three biological replicates are shown. Blue, <t>DAPI;</t> green, EdU. Scale bar = 20 µm. i CX-5461 induced DNA damage (γ-H2AX, red) was enriched in cells with active DNA synthesis (positive for EdU (green) labeling). Representative results of three biological replicates are shown. Scale bar = 10 μm. Two-tailed t- test was used for (b, c, g). Source data for these panels are included in Source Data file.
Dapi, Cell Viability And Cytotoxicity Assay Reagents, supplied by Beyotime, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dapi, cell viability and cytotoxicity assay reagents/product/Beyotime
Average 90 stars, based on 1 article reviews
dapi, cell viability and cytotoxicity assay reagents - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
perfusion with 4% paraformaldehyde pfa  (Nacalai)
90
Nacalai perfusion with 4% paraformaldehyde pfa
Fig. 3 CX-5461 causes DNA damage and cell death by apoptosis in neuroblastoma cells at sub-micromolar concentrations. a Apoptosis in CHP-134 cells. The cells were treated for 48 h. b Quantification of data in (a). Data represent the mean ± SD of three independent experiments. Significant P-values (left to right): 3.9 × 10−6, 7.2 × 10−7, and 4.8 × 10−7. c Quantification of flow cytometry data showing apoptosis up to 48 h in CHP-134 and IMR-5 (0.2 µM and 0.1 µM of CX-5461 respectively). CHP-134 cells were treated with 0.2 µM and IMR-5 cells with 0.1 µM of CX-5461. Data represent the mean ± SD of three independent experiments. Significant P-values (left to right): CHP-134, 2.2 × 10−4, 3.2 × 10−5, and 1.5 × 10−5; IMR-5, 1.8 × 10−4, 2.3 × 10−5, and 2.5 × 10−6. d Western blots for cleaved PARP (c-PARP). β-Actin was used as a loading control. Representative results of three biological replicates are shown. e Co-localization of 53BP1 and γ-H2AX. Cells were treated for 24 h. Representative results of three biological replicates are shown. Scale bar = 10 μm. See Fig. S3d for more time points. f Comet assay in CHP-134 cells. Tail length (left) and representative images (right) are shown. The red line indicates the mean value of tail length in each group. Scale bar = 5 µm. g Cell cycle analysis forCHP-134 (0.2 µM CX-5461) and IMR-5 (0.05 µM CX-5461) cells. Data represent the mean ± SD of three independent experiments. P-values vs 0 h: P1 = 4.2 × 10−5, P2 = 7.1 × 10−7, P3 = 6.3 × 10−5, P4 = 2.7 × 10−4, P5 = 6.3 × 10−3, P6 = 2.3 × 10−5, P7 = 1.8 × 10−4, and P8 = 0.044. h EdU incorporation assay to assess global nascent DNA transcription. Representative results of three biological replicates are shown. Blue, <t>DAPI;</t> green, EdU. Scale bar = 20 µm. i CX-5461 induced DNA damage (γ-H2AX, red) was enriched in cells with active DNA synthesis (positive for EdU (green) labeling). Representative results of three biological replicates are shown. Scale bar = 10 μm. Two-tailed t- test was used for (b, c, g). Source data for these panels are included in Source Data file.
Perfusion With 4% Paraformaldehyde Pfa, supplied by Nacalai, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/perfusion with 4% paraformaldehyde pfa/product/Nacalai
Average 90 stars, based on 1 article reviews
perfusion with 4% paraformaldehyde pfa - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
prolonged gold anti-fade with dapi  (Beijing Solarbio Science)
90
Beijing Solarbio Science prolonged gold anti-fade with dapi
Fig. 3 CX-5461 causes DNA damage and cell death by apoptosis in neuroblastoma cells at sub-micromolar concentrations. a Apoptosis in CHP-134 cells. The cells were treated for 48 h. b Quantification of data in (a). Data represent the mean ± SD of three independent experiments. Significant P-values (left to right): 3.9 × 10−6, 7.2 × 10−7, and 4.8 × 10−7. c Quantification of flow cytometry data showing apoptosis up to 48 h in CHP-134 and IMR-5 (0.2 µM and 0.1 µM of CX-5461 respectively). CHP-134 cells were treated with 0.2 µM and IMR-5 cells with 0.1 µM of CX-5461. Data represent the mean ± SD of three independent experiments. Significant P-values (left to right): CHP-134, 2.2 × 10−4, 3.2 × 10−5, and 1.5 × 10−5; IMR-5, 1.8 × 10−4, 2.3 × 10−5, and 2.5 × 10−6. d Western blots for cleaved PARP (c-PARP). β-Actin was used as a loading control. Representative results of three biological replicates are shown. e Co-localization of 53BP1 and γ-H2AX. Cells were treated for 24 h. Representative results of three biological replicates are shown. Scale bar = 10 μm. See Fig. S3d for more time points. f Comet assay in CHP-134 cells. Tail length (left) and representative images (right) are shown. The red line indicates the mean value of tail length in each group. Scale bar = 5 µm. g Cell cycle analysis forCHP-134 (0.2 µM CX-5461) and IMR-5 (0.05 µM CX-5461) cells. Data represent the mean ± SD of three independent experiments. P-values vs 0 h: P1 = 4.2 × 10−5, P2 = 7.1 × 10−7, P3 = 6.3 × 10−5, P4 = 2.7 × 10−4, P5 = 6.3 × 10−3, P6 = 2.3 × 10−5, P7 = 1.8 × 10−4, and P8 = 0.044. h EdU incorporation assay to assess global nascent DNA transcription. Representative results of three biological replicates are shown. Blue, <t>DAPI;</t> green, EdU. Scale bar = 20 µm. i CX-5461 induced DNA damage (γ-H2AX, red) was enriched in cells with active DNA synthesis (positive for EdU (green) labeling). Representative results of three biological replicates are shown. Scale bar = 10 μm. Two-tailed t- test was used for (b, c, g). Source data for these panels are included in Source Data file.
Prolonged Gold Anti Fade With Dapi, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/prolonged gold anti-fade with dapi/product/Beijing Solarbio Science
Average 90 stars, based on 1 article reviews
prolonged gold anti-fade with dapi - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
prolong diamon antifade mountant with dapi reagent  (Diamon Gmbh)
90
Diamon Gmbh prolong diamon antifade mountant with dapi reagent
Fig. 3 CX-5461 causes DNA damage and cell death by apoptosis in neuroblastoma cells at sub-micromolar concentrations. a Apoptosis in CHP-134 cells. The cells were treated for 48 h. b Quantification of data in (a). Data represent the mean ± SD of three independent experiments. Significant P-values (left to right): 3.9 × 10−6, 7.2 × 10−7, and 4.8 × 10−7. c Quantification of flow cytometry data showing apoptosis up to 48 h in CHP-134 and IMR-5 (0.2 µM and 0.1 µM of CX-5461 respectively). CHP-134 cells were treated with 0.2 µM and IMR-5 cells with 0.1 µM of CX-5461. Data represent the mean ± SD of three independent experiments. Significant P-values (left to right): CHP-134, 2.2 × 10−4, 3.2 × 10−5, and 1.5 × 10−5; IMR-5, 1.8 × 10−4, 2.3 × 10−5, and 2.5 × 10−6. d Western blots for cleaved PARP (c-PARP). β-Actin was used as a loading control. Representative results of three biological replicates are shown. e Co-localization of 53BP1 and γ-H2AX. Cells were treated for 24 h. Representative results of three biological replicates are shown. Scale bar = 10 μm. See Fig. S3d for more time points. f Comet assay in CHP-134 cells. Tail length (left) and representative images (right) are shown. The red line indicates the mean value of tail length in each group. Scale bar = 5 µm. g Cell cycle analysis forCHP-134 (0.2 µM CX-5461) and IMR-5 (0.05 µM CX-5461) cells. Data represent the mean ± SD of three independent experiments. P-values vs 0 h: P1 = 4.2 × 10−5, P2 = 7.1 × 10−7, P3 = 6.3 × 10−5, P4 = 2.7 × 10−4, P5 = 6.3 × 10−3, P6 = 2.3 × 10−5, P7 = 1.8 × 10−4, and P8 = 0.044. h EdU incorporation assay to assess global nascent DNA transcription. Representative results of three biological replicates are shown. Blue, <t>DAPI;</t> green, EdU. Scale bar = 20 µm. i CX-5461 induced DNA damage (γ-H2AX, red) was enriched in cells with active DNA synthesis (positive for EdU (green) labeling). Representative results of three biological replicates are shown. Scale bar = 10 μm. Two-tailed t- test was used for (b, c, g). Source data for these panels are included in Source Data file.
Prolong Diamon Antifade Mountant With Dapi Reagent, supplied by Diamon Gmbh, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/prolong diamon antifade mountant with dapi reagent/product/Diamon Gmbh
Average 90 stars, based on 1 article reviews
prolong diamon antifade mountant with dapi reagent - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
dapi staining reagent  (Logos Biosystems Inc)
90
Logos Biosystems Inc dapi staining reagent
Fig. 3 CX-5461 causes DNA damage and cell death by apoptosis in neuroblastoma cells at sub-micromolar concentrations. a Apoptosis in CHP-134 cells. The cells were treated for 48 h. b Quantification of data in (a). Data represent the mean ± SD of three independent experiments. Significant P-values (left to right): 3.9 × 10−6, 7.2 × 10−7, and 4.8 × 10−7. c Quantification of flow cytometry data showing apoptosis up to 48 h in CHP-134 and IMR-5 (0.2 µM and 0.1 µM of CX-5461 respectively). CHP-134 cells were treated with 0.2 µM and IMR-5 cells with 0.1 µM of CX-5461. Data represent the mean ± SD of three independent experiments. Significant P-values (left to right): CHP-134, 2.2 × 10−4, 3.2 × 10−5, and 1.5 × 10−5; IMR-5, 1.8 × 10−4, 2.3 × 10−5, and 2.5 × 10−6. d Western blots for cleaved PARP (c-PARP). β-Actin was used as a loading control. Representative results of three biological replicates are shown. e Co-localization of 53BP1 and γ-H2AX. Cells were treated for 24 h. Representative results of three biological replicates are shown. Scale bar = 10 μm. See Fig. S3d for more time points. f Comet assay in CHP-134 cells. Tail length (left) and representative images (right) are shown. The red line indicates the mean value of tail length in each group. Scale bar = 5 µm. g Cell cycle analysis forCHP-134 (0.2 µM CX-5461) and IMR-5 (0.05 µM CX-5461) cells. Data represent the mean ± SD of three independent experiments. P-values vs 0 h: P1 = 4.2 × 10−5, P2 = 7.1 × 10−7, P3 = 6.3 × 10−5, P4 = 2.7 × 10−4, P5 = 6.3 × 10−3, P6 = 2.3 × 10−5, P7 = 1.8 × 10−4, and P8 = 0.044. h EdU incorporation assay to assess global nascent DNA transcription. Representative results of three biological replicates are shown. Blue, <t>DAPI;</t> green, EdU. Scale bar = 20 µm. i CX-5461 induced DNA damage (γ-H2AX, red) was enriched in cells with active DNA synthesis (positive for EdU (green) labeling). Representative results of three biological replicates are shown. Scale bar = 10 μm. Two-tailed t- test was used for (b, c, g). Source data for these panels are included in Source Data file.
Dapi Staining Reagent, supplied by Logos Biosystems Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dapi staining reagent/product/Logos Biosystems Inc
Average 90 stars, based on 1 article reviews
dapi staining reagent - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier

Image Search Results


Fig. 3 CX-5461 causes DNA damage and cell death by apoptosis in neuroblastoma cells at sub-micromolar concentrations. a Apoptosis in CHP-134 cells. The cells were treated for 48 h. b Quantification of data in (a). Data represent the mean ± SD of three independent experiments. Significant P-values (left to right): 3.9 × 10−6, 7.2 × 10−7, and 4.8 × 10−7. c Quantification of flow cytometry data showing apoptosis up to 48 h in CHP-134 and IMR-5 (0.2 µM and 0.1 µM of CX-5461 respectively). CHP-134 cells were treated with 0.2 µM and IMR-5 cells with 0.1 µM of CX-5461. Data represent the mean ± SD of three independent experiments. Significant P-values (left to right): CHP-134, 2.2 × 10−4, 3.2 × 10−5, and 1.5 × 10−5; IMR-5, 1.8 × 10−4, 2.3 × 10−5, and 2.5 × 10−6. d Western blots for cleaved PARP (c-PARP). β-Actin was used as a loading control. Representative results of three biological replicates are shown. e Co-localization of 53BP1 and γ-H2AX. Cells were treated for 24 h. Representative results of three biological replicates are shown. Scale bar = 10 μm. See Fig. S3d for more time points. f Comet assay in CHP-134 cells. Tail length (left) and representative images (right) are shown. The red line indicates the mean value of tail length in each group. Scale bar = 5 µm. g Cell cycle analysis forCHP-134 (0.2 µM CX-5461) and IMR-5 (0.05 µM CX-5461) cells. Data represent the mean ± SD of three independent experiments. P-values vs 0 h: P1 = 4.2 × 10−5, P2 = 7.1 × 10−7, P3 = 6.3 × 10−5, P4 = 2.7 × 10−4, P5 = 6.3 × 10−3, P6 = 2.3 × 10−5, P7 = 1.8 × 10−4, and P8 = 0.044. h EdU incorporation assay to assess global nascent DNA transcription. Representative results of three biological replicates are shown. Blue, DAPI; green, EdU. Scale bar = 20 µm. i CX-5461 induced DNA damage (γ-H2AX, red) was enriched in cells with active DNA synthesis (positive for EdU (green) labeling). Representative results of three biological replicates are shown. Scale bar = 10 μm. Two-tailed t- test was used for (b, c, g). Source data for these panels are included in Source Data file.

Journal: Nature communications

Article Title: The chemotherapeutic CX-5461 primarily targets TOP2B and exhibits selective activity in high-risk neuroblastoma.

doi: 10.1038/s41467-021-26640-x

Figure Lengend Snippet: Fig. 3 CX-5461 causes DNA damage and cell death by apoptosis in neuroblastoma cells at sub-micromolar concentrations. a Apoptosis in CHP-134 cells. The cells were treated for 48 h. b Quantification of data in (a). Data represent the mean ± SD of three independent experiments. Significant P-values (left to right): 3.9 × 10−6, 7.2 × 10−7, and 4.8 × 10−7. c Quantification of flow cytometry data showing apoptosis up to 48 h in CHP-134 and IMR-5 (0.2 µM and 0.1 µM of CX-5461 respectively). CHP-134 cells were treated with 0.2 µM and IMR-5 cells with 0.1 µM of CX-5461. Data represent the mean ± SD of three independent experiments. Significant P-values (left to right): CHP-134, 2.2 × 10−4, 3.2 × 10−5, and 1.5 × 10−5; IMR-5, 1.8 × 10−4, 2.3 × 10−5, and 2.5 × 10−6. d Western blots for cleaved PARP (c-PARP). β-Actin was used as a loading control. Representative results of three biological replicates are shown. e Co-localization of 53BP1 and γ-H2AX. Cells were treated for 24 h. Representative results of three biological replicates are shown. Scale bar = 10 μm. See Fig. S3d for more time points. f Comet assay in CHP-134 cells. Tail length (left) and representative images (right) are shown. The red line indicates the mean value of tail length in each group. Scale bar = 5 µm. g Cell cycle analysis forCHP-134 (0.2 µM CX-5461) and IMR-5 (0.05 µM CX-5461) cells. Data represent the mean ± SD of three independent experiments. P-values vs 0 h: P1 = 4.2 × 10−5, P2 = 7.1 × 10−7, P3 = 6.3 × 10−5, P4 = 2.7 × 10−4, P5 = 6.3 × 10−3, P6 = 2.3 × 10−5, P7 = 1.8 × 10−4, and P8 = 0.044. h EdU incorporation assay to assess global nascent DNA transcription. Representative results of three biological replicates are shown. Blue, DAPI; green, EdU. Scale bar = 20 µm. i CX-5461 induced DNA damage (γ-H2AX, red) was enriched in cells with active DNA synthesis (positive for EdU (green) labeling). Representative results of three biological replicates are shown. Scale bar = 10 μm. Two-tailed t- test was used for (b, c, g). Source data for these panels are included in Source Data file.

Article Snippet: ProLong® Gold Antifade Reagent with DAPI (Cell Signaling, #8961) was used as coverslip mountant.

Techniques: Cytometry, Western Blot, Control, Single Cell Gel Electrophoresis, Cell Cycle Assay, DNA Synthesis, Labeling, Two Tailed Test